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il-6 promoter construct  (Active Motif)


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    Active Motif il-6 promoter construct
    Il 6 Promoter Construct, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il-6+promoter+construct/il+6+promoter+construct/pmc04742771-152-16-25
    Average 90 stars, based on 1 article reviews
    il-6 promoter construct - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Luciferase:

    Article Title: Ras Regulates Rb via NORE1A
    Article Snippet: Luciferase assays were performed in triplicate using the LightSwitch TM luciferase assay kit with a corresponding IL-6 promoter construct (stock keeping unit S721728) generated by Active Motif (Carlsbad, CA).

    Construct:

    Article Title: Ras Regulates Rb via NORE1A
    Article Snippet: Luciferase assays were performed in triplicate using the LightSwitch TM luciferase assay kit with a corresponding IL-6 promoter construct (stock keeping unit S721728) generated by Active Motif (Carlsbad, CA).

    Generated:

    Article Title: Ras Regulates Rb via NORE1A
    Article Snippet: Luciferase assays were performed in triplicate using the LightSwitch TM luciferase assay kit with a corresponding IL-6 promoter construct (stock keeping unit S721728) generated by Active Motif (Carlsbad, CA).

    Western Blot:

    Article Title: Ras Regulates Rb via NORE1A
    Article Snippet: Luciferase assays were performed in triplicate using the LightSwitch TM luciferase assay kit with a corresponding IL-6 promoter construct (stock keeping unit S721728) generated by Active Motif (Carlsbad, CA).

    Immunoprecipitation:

    Article Title: Ras Regulates Rb via NORE1A
    Article Snippet: Luciferase assays were performed in triplicate using the LightSwitch TM luciferase assay kit with a corresponding IL-6 promoter construct (stock keeping unit S721728) generated by Active Motif (Carlsbad, CA).



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    Image Search Results


    Schematic of MS2 constructs used in this study. Plasmid A) PGK‐MS2‐GFP fusion protein Plasmid, expresses the MS2‐GFP fusion protein; Plasmid B) reporter mRNA, expresses an MS2 × 24 repeat sequence driven by IL‐6 promoter (IL‐6‐RFP‐MS2). Single transfected cells (Plasmid A, left side) showed a diffuse green fluorescence concentrated within the cell nucleus because of the nuclear localization sequence (NLS); In co‐transfected cells (right side), the fusion protein co‐expresses with the reporter mRNA containing MS2‐binding sites. The fusion protein specifically binds to one of the 24 copies of the RNA motif contained in the MS2 × 24 repeat sequence. The resultant binding of multiple copies of the MS2‐GFP fusion protein to the mRNA forms bright green particles in cells (particularly induced by pro‐inflammatory factor IL‐1α).

    Journal: Journal of Orthopaedic Research

    Article Title: Dynamic imaging demonstrates that pulsed electromagnetic fields (PEMF) suppress IL‐6 transcription in bovine nucleus pulposus cells

    doi: 10.1002/jor.23713

    Figure Lengend Snippet: Schematic of MS2 constructs used in this study. Plasmid A) PGK‐MS2‐GFP fusion protein Plasmid, expresses the MS2‐GFP fusion protein; Plasmid B) reporter mRNA, expresses an MS2 × 24 repeat sequence driven by IL‐6 promoter (IL‐6‐RFP‐MS2). Single transfected cells (Plasmid A, left side) showed a diffuse green fluorescence concentrated within the cell nucleus because of the nuclear localization sequence (NLS); In co‐transfected cells (right side), the fusion protein co‐expresses with the reporter mRNA containing MS2‐binding sites. The fusion protein specifically binds to one of the 24 copies of the RNA motif contained in the MS2 × 24 repeat sequence. The resultant binding of multiple copies of the MS2‐GFP fusion protein to the mRNA forms bright green particles in cells (particularly induced by pro‐inflammatory factor IL‐1α).

    Article Snippet: Plasmid B: the mRNA reporter construct was generated by ligating IL‐6 promoter‐RFP‐MS2 × 24 in between NotI and HindIII sites of p miniTol2 (Addgene).

    Techniques: Construct, Plasmid Preparation, Sequencing, Transfection, Fluorescence, Binding Assay

    Visualization of dynamic mRNA expression in individual live cells. A1) Illustration of IL‐6 expression ON and OFF in MS2‐GFP system. A2) Single GFP particles in living cells after transfection. B) GFP particle distribution in single living cells after co‐transfection (both in the cytoplasm and nucleus); White dots represent GFP particles that are tracked with Imaris software. C) Dynamic imaging of GFP in nucleus of living cells after co‐transfection (induced by IL‐1α) for 400 min. Exposure time is reduced to visualize the particulate nature of the intense nuclear stain.

    Journal: Journal of Orthopaedic Research

    Article Title: Dynamic imaging demonstrates that pulsed electromagnetic fields (PEMF) suppress IL‐6 transcription in bovine nucleus pulposus cells

    doi: 10.1002/jor.23713

    Figure Lengend Snippet: Visualization of dynamic mRNA expression in individual live cells. A1) Illustration of IL‐6 expression ON and OFF in MS2‐GFP system. A2) Single GFP particles in living cells after transfection. B) GFP particle distribution in single living cells after co‐transfection (both in the cytoplasm and nucleus); White dots represent GFP particles that are tracked with Imaris software. C) Dynamic imaging of GFP in nucleus of living cells after co‐transfection (induced by IL‐1α) for 400 min. Exposure time is reduced to visualize the particulate nature of the intense nuclear stain.

    Article Snippet: Plasmid B: the mRNA reporter construct was generated by ligating IL‐6 promoter‐RFP‐MS2 × 24 in between NotI and HindIII sites of p miniTol2 (Addgene).

    Techniques: Expressing, Transfection, Cotransfection, Software, Imaging, Staining

    Flow cytometry verifying the GFP and RFP profile in MS2‐GFP fusion and mRNA reporter system. A) Control, without transfection. B) MS2‐GFP fusion plasmid single transfection C) IL‐6‐RFP‐MS2 plasmid single transfection D) Co‐transfection ( n = 3/group). MFI = mean fluorescence intensity.

    Journal: Journal of Orthopaedic Research

    Article Title: Dynamic imaging demonstrates that pulsed electromagnetic fields (PEMF) suppress IL‐6 transcription in bovine nucleus pulposus cells

    doi: 10.1002/jor.23713

    Figure Lengend Snippet: Flow cytometry verifying the GFP and RFP profile in MS2‐GFP fusion and mRNA reporter system. A) Control, without transfection. B) MS2‐GFP fusion plasmid single transfection C) IL‐6‐RFP‐MS2 plasmid single transfection D) Co‐transfection ( n = 3/group). MFI = mean fluorescence intensity.

    Article Snippet: Plasmid B: the mRNA reporter construct was generated by ligating IL‐6 promoter‐RFP‐MS2 × 24 in between NotI and HindIII sites of p miniTol2 (Addgene).

    Techniques: Flow Cytometry, Control, Transfection, Plasmid Preparation, Cotransfection, Fluorescence